a. What is O-linked glycosylation and how do they differ from N-linked
b. Cite an example of how bacterial organisms can sometimes mimic O-linked glycosylation to confuse mammalian host cells. (brief description)
i. Focus on one bacterial organism? Virulent or commensal.
ii. What mammalian host cell do they typically influence?
iii. O-linked branched chains and Mechanism of action
2. Antibody-drug conjugates are the most recent advancement in current
a. Brief explanation of what these are?
b. Provide an example: either currently being researched or on the market
c. How are these conjugated to the drug – counterparts? What kind of bonds are used? (Brief explanation)
d. Provide a Schematic or Diagram of a process chain with a brief explanation for each process within the chain.
3. The expression of Rv2629 modulates the growth of M. smegmatis under aerobic conditions. The growth curve of the recombinant strains exhibits reduced expression or in some cases over expression, which is influencing the growth rates of specific strains. The cell growth is being measured using turbidity (optical density counts) at 600 nm absorbance values. The abbreviations were: MS (wild type), MSP (control strain transformed with the pMV261 plasmid), and MSpACT (control strain transformed with the pACT plasmid). The results indicated (A) comparatively slow growth of MSW. (B) Comparatively rapid growth of MSL;
Reference: Dan Liu et al Front. Microbiol., 15 November 2017, https://doi.org/10.3389/fmicb.2017.02231
a. From the graph – interpret which 3 growth phases are being studied here.
b. Which phase is being influenced the most?
c. State 3 reasons for why this particular phase is important
4- WHAT ARE SOME IMPORTANT CHARACTERISTICS OF AN APPROPRIATE HOST CELL LINE FOR BIOPROCESS DESIGN FOR MONOCLONAL ANTIBODY PRODUCTION FOR THE TREATMENT OF CANCER?
5- HOW DO ENZYMES INFLUENCE THE REACTION ENERGY IN A CATALYSIS PROCESS AND WHAT ARE SOME KEY DIFFERENCES BETWEEN RAPID -EQUILIBRIUM VERSUS QUASI-STEADY STATE ASSUMPTIONS OF ENZYME KINETICS?
6- WHICH METABOLIC PATHWAY CONTROL MECHANISM PLAYS A MAJOR ROLE IN THE REGULATION OF THE PROLINE-ARGININE (KEGG) PATHWAY? BRIEFLY DISCUSS THIS SPECIFIC METABOLIC CONTROL MECHANISM.
7- AEROBIC GROWTH OF S. CEREVISIAE ON ETHANOL IS SIMPLY DESCRIBED BY THE FOLLOWING OVERALL REACTION
C2H50H + a 0 2 + b NH3 cCH1.704 N0.149 00.408 + d CO 2 + e H20.
(a) Determine the coefficients a, b, c and d, where RQ = 0.66
(b) Determine the biomass yield coefficient (gdw/substrate), and the oxygen yield coefficient (in gdw/O2)
Please cite references when used.